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Marijuana testing TM

Organic medicine for the Planet

 

LABORATORY TESTING

 

 

  • Labs do different types of testing- Gas chromatography (GC), High-performance liquid chromatography,(HPLC )and Thin Layer Chromatography ( TLC) or somtimes called (HPTLC)(* which is what our kit does - see descripitions below)*), They first have to be DEA approved to not be illegal, next the products they test have to be by registered growers, finally the BIG ONE is that there testing has not been proven to be any better than these kits, the same plant can be tested 3 times and get 3 different results, also if you took the same sample to three different labs you would get three different results.
  • Basically there is no standard procedure from lab to lab for testing Medical Marijuana because of the lack of need until now.
  • Without another type of test , Suppliers will be required to pay high prices for testing in Labs which may be hard to reach and still get varied results, when they can do in- house testing at a ** Fraction of the Cost** and hassle.

 

3 Most common types of Laboratory Testing

 

  • Thin Layer Chromatography (TLC) – or sometimes called (HPTLC) **(Type of kit we offer -see method of testing below)
  • Thin layer chromatography (TLC) is a widely-used chromatography technique used to separate chemical compounds. It involves a stationary phase consisting of a thin layer of adsorbent material, usually silica gel, aluminum oxide, or cellulose immobilized onto a flat, inert carrier sheet. A liquid phase consisting of the solution to be separated dissolved in an appropriate solvent is drawn through the plate via capillary action, separating the experimental solution, whereas TLC can be configured for quantitative work, at Galbraith it is primarily used in identification tests of plants and other materials.

 

  • High Performance Liquid Chromatography (HPLC)
  • High-performance liquid chromatography is a form of column chromatography used frequently in analytical chemistry. HPLC is used to separate components of a mixture by using a variety of chemical interactions between the compounds within the substance being analyzed and the chromatography column.

 

  • Gas Chromatography (GC)
  • A Gas Chromatograph is a chemical analysis instrument used for separating volatile and semi-volatile compounds in a complex sample. The stationary phase in GC is contained in a narrow -bored column and the mobile phase is a gas. The volatile and semi-volatile components of a sample mixture are separated based on their varying partition behavior between the mobile phase and the stationary phase.

The Reality is GC testing is not good for testing of CBD, CBN,CBG, and some of the other substances in marijuana because the machines use heat to test these substances and they all evaporate at a lower temperature then THC. So you will get some results but they will not be that accurate

HPLC testing is complex and does do cold testing but if you are looking for the highest THC% then you must test HOT & COLD which most machines are not prepared to do. Also calibrating the machines should be done after every batch is tested and that is not done, all these systems need expensive equipment and are costly.

 

A in depth technical Comparison of the above Testing Methods Click Here

 

 

 

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Here is a sample of some plates that were done making 5 lanes, The one on the left is a cold plate test, the one on the right is a hot plate test. These are examples: The CBD & CBG are brighter then can be seen here.

Remember "Cold testing" which shows all the acids and the other relevant substances in the product up through the THC and CBD,CBN,CBG etc.

"Hot testing " which moves more of the THC up to the top by dissolving some of the substances that would be left when a cold test is done, By heating the sample spot it will evaporate some of the CBD CBN and other substances because they evaporate at a lower temperature then THC does.

The bottom line is, highest THC % is tested by using the "hot testing method " For results of all substances relevant to each other you use "cold testing method "

 

Method of Testing with our Kits:

NOTE: Our test can do both hot and cold testing (see the video ) the difference is you heat the spot if you want high THC% value or leave it un heated if you want the cold value BEFORE putting the plate in the developing jar.( There is more information in the custom manual we supply)

TLC or “Thin Layer Chromatography” is a simple, quick, common and inexpensive procedure that gives a quick answer as to how many components are in a mixture. TLC is also used to support the identity of a compound in a mixture when the Rf of a compound is compared with the Rf from the literature, In our case the cannabinoids can be identified by their specific color and specific location (Rf value) and order of appearance on the TLC plate, if carried out with the solvent system from the Kit.
A TLC plate is a sheet of glass which is coated with a thin layer of a solid adsorbent, silica. A small amount of the mixture to be analyzed is spotted near the bottom of this plate. The TLC plate is then placed in a shallow pool of a solvent in a developing chamber so that only the very bottom of the plate is in the liquid. This liquid element or carrier fluid, is the mobile phase, and it slowly rises up the TLC plate by capillary action.

As the solvent moves, it will pass the little spot of cannabinoid-oil micro-droplet of 2 ul that was applied on the bottom of the plate. An equilibrium is established for each component of the mixture between the molecules of that component which are absorbed on the solid and the molecules which are in solution. In principle, the components will differ in solubility and in strength of their adsorption to the adsorbent and the other components will be carried farther up to the plate than the others. When the solvent has reached the top of the plate, the plate is removed from the developing chamber, dried (ventilation!) . After drying, the separated components (cannabinoids) now separated in the silica plates, will be visualized by spraying the plate with a dye. The dye is highly specific for cannabinoids. Every principal cannabinoid will display its own color! THC: wine red, CBD: bright yellow, CBG: orange, CBN: violet etc..

Independent Research of Our Testing Procedure

 

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TLC - What is a Retention Factor (Rf)

The retention factor, or Rf, is defined as the distance traveled by the compound divided by the distance traveled by the solvent.

For example, if a compound travels 2.1 cm and the solvent front travels 2.8 cm, the Rf is 0.75:

The Rf for a compound is a constant from one experiment to the next only if the chromatography conditions below are also constant:

solvent system

adsorbent

thickness of the adsorbent

amount of material spotted

temperature

Since these factors are difficult to keep constant from experiment to experiment, relative Rf values are generally considered. “Relative Rf” means that the values are reported relative to a standard, or it means that you compare the Rf values of compounds run on the same plate at the same time.

The larger an Rf of a compound, the larger the distance it travels on the TLC plate. When comparing two different compounds run under identical chromatography conditions, the compound with the larger Rf is less polar because it interacts less strongly with the polar adsorbent on the TLC plate. Conversely, if you know the structures of the compounds in a mixture, you can predict that a compound of low polarity will have a larger Rf value than a polar compound run on the same plate.

The Rf can provide corroborative evidence as to the identity of a compound. If the identity of a compound is suspected but not yet proven, an authentic sample of the compound, or standard, is spotted and run on a TLC plate side by side (or on top of each other) with the compound in question. If two substances have the same Rf value, they are likely (but not necessarily) the same compound. If they have different Rf values, they are definitely different compounds. Note that this identity check must be performed on a single plate, because it isdifficult to duplicate all the factors which influence Rf exactly from experiment to experiment.

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